Composite

Part:BBa_K4737021:Design

Designed by: Peisong Wu   Group: iGEM23_BNUZH-China   (2023-10-04)


prpsM::HlyA-6xHis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal PstI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal PstI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal PstI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

The BNUZH-China iGEM team 2023 designed and built.

References

Domann E, Chakraborty T. Nucleotide sequence of the listeriolysin gene from a Listeria monocytogenes serotype 1/2a strain. Nucleic Acids Res. 1989 Aug 11;17(15):6406. doi: 10.1093/nar/17.15.6406. PMID: 2505236; PMCID: PMC318305.